Electroconductive nanopatterned substrates for enhanced myogenic differentiation and maturation.
최종 수정일: 2021년 1월 29일
ADVANCED HEALTHCARE MATERIALS
Volume5, Issue1, January 7, 2016, Pages 1-184
Impact Factor : 7.367
Hee Seok Yang , Bora Lee , Jonathan H. Tsui , Jesse Macadangdang , Seok-Young Jang ,
Sung Gap Im , * and Deok-Ho Kim *
Electrically conductive materials provide a suitable platform for the in vitro study of excitable cells, such as skeletal muscle cells, due to their inherent conductivity and electroactivity. Here it is demonstrated that bioinspired electroconductive nanopatterned substrates enhance myogenic differentiation and maturation. The topographical cues from the highly aligned collagen bundles that form the extracellular matrix of skeletal muscle tissue are mimicked using nanopatterns created with capillary force lithography. Electron beam deposition is then utilized to conformally coat nanopatterned substrates with a thin layer of either gold or titanium to create electroconductive substrates with well-defi ned, large-area nanotopographical features. C2C12 cells, a myoblast cell line, are cultured for 7 d on substrates and the effects of topography and electrical conductivity on cellular morphology and myogenic differentiation are assessed. It is found that biomimetic nanotopography enhances the formation of aligned myotubes and the addition of an electroconductive coating promotes myogenic differentiation and maturation, as indicated by the upregulation of myogenic regulatory factors Myf5 , MyoD , and myogenin ( MyoG ). These results suggest the suitability of electroconductive nanopatterned substrates as a biomimetic platform for the in vitro engineering of skeletal muscle tissue.